A Study of a Possible New Vaccine Candidate rWbL2 In Lymphatic Filariasis In Mastomys ; An Animal Study
Keywords:
Wuchereria bancrofti, Brugia malayi, SXP/RAL-2 family protein WbL2, vaccine, mastomysAbstract
Lymphatic filariasis is a major health problem caused mainly by three lymph dwelling parasites; Wuchereria bancrofti, Brugia malayi and Brugia timori. Since long it has been cause of clinical morbidity in tropical and subtropical countries. Currently there is no vaccine available to prevent this infection. For complete elimination of filariasis, vaccine could complement the existing therapeutic and vector control methods. In the present study, in an attempt to explore the efficacy of a vaccine candidate, we have shown that the mastomys, which are fully permissible rodents for Brugia malayi infection when immunized with Wuchereria bancrofti bancrofti recombinant SXP/RAL-2 family protein L2 (rWbL2) could induce 51.25% in situ cytotoxicity against Brugia malayi infective (L3) larvae. The protection was characterized by higher levels of WbL2- specific IgG and IgG2a antibodies and significant levels of IFN-y, caninum has already been successfully tested as a IL-4 and IL-10 cytokines .The findings indicate that immunization of mastomys with rWbL2 could impart protection against filarial infection.
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